Review



azdye546 picolyl azide  (Jena Bioscience)


Bioz Verified Symbol Jena Bioscience is a verified supplier
Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Jena Bioscience azdye546 picolyl azide
    Azdye546 Picolyl Azide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/azdye546+azide/AZDye546-Picolyl-Azide/bio_rxiv__64898__2026__04__29__721357-209-22-23
    Average 94 stars, based on 2 article reviews
    azdye546 picolyl azide - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Chlamydia trachomatis deploys sphingolipids for genome organisation
    Article Snippet: .. Subsequently, alkyne groups of TFSM derivatives were stained with AZDye546 azide [Jena Bioscience, Cat. No. CLK-1283; product discontinued and we recommend using AZDye546-picolyl-azide (Jena Bioscience, Cat. No. CLK-1284) alternatively] for 1 h/37°C at 170 rpm. ..



    Similar Products

    azdye546 picolyl azide  (Jena Bioscience)
    94
    Jena Bioscience azdye546 picolyl azide
    Azdye546 Picolyl Azide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/azdye546+azide/AZDye546-Picolyl-Azide/bio_rxiv__64898__2026__04__29__721357-209-22-23
    Average 94 stars, based on 1 article reviews
    azdye546 picolyl azide - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    azdye546 azide  (Jena Bioscience)
    94
    Jena Bioscience azdye546 azide
    HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and <t>AZDye546-azide,</t> 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.
    Azdye546 Azide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/azdye546+azide/AZDye546-Azide/bio_rxiv__64898__2026__04__29__721357-209-9-11
    Average 94 stars, based on 1 article reviews
    azdye546 azide - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and AZDye546-azide, 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.

    Journal: bioRxiv

    Article Title: Chlamydia trachomatis deploys sphingolipids for genome organisation

    doi: 10.64898/2026.04.29.721357

    Figure Lengend Snippet: HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and AZDye546-azide, 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.

    Article Snippet: Subsequently, alkyne groups of TFSM derivatives were stained with AZDye546 azide [Jena Bioscience, Cat. No. CLK-1283; product discontinued and we recommend using AZDye546-picolyl-azide (Jena Bioscience, Cat. No. CLK-1284) alternatively] for 1 h/37°C at 170 rpm.

    Techniques: Infection, Expressing, Quantitative RT-PCR, Activity Assay, Incubation, Staining, Comparison